in press
1.Matsumoto Y, Miyazaki S, Fukunaga D, Shimizu K, Kawamoto S, Sekimizu K. Quantitative evaluation of cryptococcal pathogenesis and antifungal drugs using a
silkworm infection model with Cryptococcus neoformans.
J Appl Microbiol. in press (2011)
2.Shinya Miyazaki, Yasuhiko Matsumoto, Kazuhisa Sekimizu, Chikara Kaito. Evaluation of Staphylococcus aureus virulence factors using a silkworm model
FEMS Microbiol Lett. in press (2011)
3.Hanada Y, Sekimizu K, Kaito C. Silkworm apolipophorin inhibits Staphylococcus aureus virulence.
J Biol Chem. in press (2011)
4.Atmika Paudel, Hiroshi Hamamoto, Yoshihisa Kobayashi, Satoshi Yokoshima, Tohru Fukuyama, and Kazuhisa Sekimizu. Identification of novel deoxyribofuranosyl indole antimicrobial agents
Journal of Antibiotics. in press (2011)
2011
I. 原著論文
1.Kaito C, Usui K, Kyuma T, Sekimizu K
Isolation of mammalian pathogenic bacteria using silkworms.
Drug Discov Ther.5(2):66-70.(2011)
2.Dhital S, Hamamoto H, Urai M, Ishii K, Sekimizu K
Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay.
Drug Discov Ther.5(1):18-25. (2011)
3.Ueda T, Kaito C, Omae Y, Sekimizu K. Sugar-responsive gene expression and the
agr system are required for colony spreading in Staphylococcus aureus.
Microb
Pathog.51(3):178-85. (2011)
4.Morishita D, Takami M, Yoshikawa S, Katayama R, Sato S, Kukimoto-Niino M,
Umehara T, Shirouzu M, Sekimizu K, Yokoyama S, Fujita N. Cell-permeable
carboxyl-terminal p27(Kip1) peptide exhibits anti-tumor activity by inhibiting
Pim-1 kinase.
J Biol Chem.286(4):2681-8. (2011)
5.Fukuyama N, Ino C, Suzuki Y, Kobayashi N, Hamamoto H, Sekimizu K, Orihara Y. Antimicrobial sesquiterpenoids from Laurus nobilis L.
Nat Prod Res.1-9. (2011)
6. Chikara Kaito, Yuki Saito, Gentaro Nagano, Mariko Ikuo, Yosuke Omae, Yuichi Hanada, Xiao Han, Kyoko Kuwahara-Arai, Tomomi Hishinuma, Tadashi Baba, Teruyo Ito, Keiichi Hiramatsu, Kazuhisa Sekimizu. Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus Virulence
PLoS pathog. 7(2): e1001267 (2011)
7.Arimitsu N, Kogure T, Baba T, Nakao K, Hamamoto H, Sekimizu K, Yamamoto A,
Nakanishi H, Taguchi R, Tagaya M, Tani K. p125/Sec23-interacting protein
(Sec23ip) is required for spermiogenesis.
FEBS Lett.585(14):2171-6. (2011)
8.Katsutoshi Imamura, Kenichi Ishii, Hiroshi Hamamoto, Kazuhisa Sekimizu. Hemocytes and humoral factors in silkworm blood are cooperatively involved in sheep erythrocyte aggregation.
Drug Discoveries & Therapeutics.5(3):125-9 (2011)
9.Matsumoto Y, Sumiya E, Sugita T, Sekimizu K. An invertebrate hyperglycemic model for identification of anti-diabetic drugs.
PLoS ONE6(3):e18292 (2011)
2010
I. 原著論文
1.Fujiyuki T, Imamura K, Hamamoto H, Sekimizu K
Evaluation of therapeutic effects and pharmacokinetics of antibacterial chromogenic agents in a silkworm model of Staphylococcus aureus infection.
Drug Discov Ther.4(5):349-354. (2010)
2.Tano K, Hamamoto H, Ito T, Sumiya E, Rakwal R, Shibato J, Masuo Y, Ijiri K, Sekimizu K, Akimitsu N
Reduced expression of Sytl 1 and Ccdc21 and impaired induction of Mt I by oxidative stress in SII-K1 knockout mice.
Drug Discov Ther.4(5):368-372. (2010)
3.Wang DL, Qi FH, Xu HL, Inagaki Y, Orihara Y, Sekimizu K, Kokudo N, Wang FS,
Tang W. Apoptosis-inducing activity of compounds screened and characterized from
cinobufacini by bioassay-guided isolation.
Mol Med Report.3(4):717-22. (2010)
4. Fujiyuki T, Imamura K, Hamamoto H, Sekimizu K. Evaluation of therapeutic effects and pharmacokinetics of antibacterial chromogenic agents in silkworm model of Staphilococcus aureus infection
Drug Discov Ther. 4(5):349-354. (2010)
5. Ishii K, Hamamoto H, Imamura K, Adachi T, Shoji M, Nakayama K, Sekimizu K. Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae.
J Biol Chem.285(43): 33338-47, (2010)
6. Ishii K, Hamamoto H, Kamimura M, Nakamura Y, Noda H, Imamura K, Mita K,
Sekimizu K.The insect cytokine paralytic peptide (PP) induces cellular and humoral immune responses in the silkworm Bombyx mori.
J Biol Chem.285(37): 28635-42, (2010)
7. Ikuo M, Kaito C, Sekimizu K: The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene.
Microb Pathog.49(1-2):1-7, (2010)
8. Asami Y, Horie R, Hamamoto H, Sekimizu K:Use of silkworms for identification of drug candidates having appropriate pharmacokinetics from plant sources.
BMC Pharmacol.11;10:7, (2010)
9. Tabuchi Y, Shiratsuchi A, Kurokawa K, Gong JH, Sekimizu K, Lee BL, Nakanishi Y. Inhibitory role for D-alanylation of wall teichoic acid in activation of insect Toll pathway by peptidoglycan of Staphylococcus aureus.
J Immunol.15;185(4):2424-31, (2010)
10. Matsuo M, Kurokawa K, Lee BL, Sekimizu K. Shuttle vectors derived from pN315 for study of essential genes in Staphylococcus aureus.
Biol Pharm Bull.33(2):198-203, (2010)
11. Matsumoto Y, Xu Q, Kaito C, Farr C L, Axelrod H L, Chiu H-J, Klock H E, Knuth M W, Miller M D, Elsliger M-A, Deacon A M, Godzik A, Lesly S A, Sekimizu K, Wilsom I A: Structure of a virulence regulatory factor CvfB reveals a novel winged-helix RNA binding module.
Structure.18, 537-547, (2010)
12. Koyama H, Ueda T, Ito T, Sekimizu K. Novel RNA polymerase II mutation suppresses transcriptional fidelity and oxidative stress sensitivity in rpb9Delta yeast.
Genes Cells. 15(2):151-9. (2010)
2009
I. 原著論文
1.Shiratsuchi A, Shimizu K, Watanabe I, Hashimoto Y, Kurokawa K, Razanajatovo IM, Park KH, Park HK, Lee BL, Sekimizu K, Nakanishi Y. Auxiliary role for D-alanylated wall teichoic acid in Toll-like receptor 2-mediated survival of Staphylococcus aureus in macrophages.
Immunology. 129:268-77. (2009)
2.Kurokawa K, Mizumura H, Takaki T, Ishii Y, Ichihashi N, Lee BL, Sekimizu K. Rapid exchange of bound ADP on the Staphylococcus aureus replication initiation protein DnaA.
J Biol Chem. 284(49):34201-10. (2009)
3.Fujiyuki T, Matsuzaka E, Nakaoka T, Takeuchi H, Wakamoto A, Ohka S, Sekimizu K, Nomoto A, Kubo T. Distribution of Kakugo virus and its effects on the gene expression profile in the brain of the worker honeybee Apis mellifera L.
J Virol. 83(22):11560-8. (2009)
4.Kurokawa K, Hamamoto H, Matsuo M, Nishida S, Yamane N, Lee BL, Murakami K, Maki H, Sekimizu K. Evaluation of target specificity of antibacterial agents using Staphylococcus aureus ddlA mutants and D-cycloserine in a silkworm infection model.
Antimicrob Agents Chemother. 53(9):4025-7. (2009)
5.Yukiko Okuyama-Nishida, Nobuko Akiyama,, Giichi Sugimori, Kazuhide Nomura, Kenji Ogawa, Koichi J. Homma, Kazuhisa Sekimizu, Masafumi Tsujimoto, and Shunji Natori. Prevention of death in bacterium-infected mice by a synthetic antimicrobial peptide, L5, through activation of host immunity.
Antimicrob Agents Chemother. 53(6):2510-6. (2009)
6.Ngata M, Ito T, Arimitsu N, Koyama H, and Sekimizu K. Transcriptional arrest rerief by S-II/TFIIS during gene expression in erythroblast defferentiation.
Genes to Cells.14(3):371-80. (2009)
7.Kurokawa K, Lee H, Roh KB, Asanuma M, Kim YS, Nakyama H, Shiratsuchi A, Choi Y, Takeuchi O, Kang HJ, Dohmae N, Nakanishi Y, Akira S, Sekimizu K, Lee BL., The triacylated ATP binding cluster transporter substrate-binding lipoprotein of Staphylococcus aureus functions as a native ligand for the toll-like receptor 2.
J Biol Chem. 284(13):8406-11. (2009)
8.Takabatake N, Iseki H, Ikehara Y, Kanuka H, Yokoyama N, Sekimizu K, Igarashi I. Isolation and pathogenic characterization of an OB1 variant of Babesia rodhaini which has a glycophorin A-independent pathway to murine red blood cells.
Vet Parasitol. 159(2):97-104. (2009)
9.Usui K, Miyazaki S, Kaito C, Sekimizu K. Purification of soil bacteria exotoxin using silkworm toxicity to measure specific activity.
Microb Pathog.2009 Feb;46(2):59-62
10.Oku Y, Kurokawa K, Matsuo M, Yamada S, Lee BL, Sekimizu K. Pleiotropic roles of poly-glycerophosphate synthase of lipoteichoic acid in the growth of Staphylococcus aureus cells.
J. Bacteriol. 191:141-51. (2009)
11.Hamamoto H, Tonoike A, Narushima K, Horie R, Sekimizu K. Sikworm as a model animal to evaluate drug candidate toxity and metabolism. Comp Biochem Physiol C. 149(3):334-9. (2009)
II. 総説
浜本洋、村上和久、関水和久 「カイコ感染モデルを利用し、治療効果を指標とした新規抗菌薬探索法の確立」
化学療法の領域 25, 2134-2140, (2009)
2008
I. 原著論文
1. Chikara Kaito, Yosuke Omae, Yasuhiko Matsumoto, Makiko Nagata, Hiroki Yamaguchi, Taiji Aoto, Teruyo Ito, Keiichi Hiramatsu, Kazuhisa Sekimizu., A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus.
PLoS ONE. 3(12):e3921 (2008)
2. Hiroshi Koyama, Eriko Sumiya, Makiko Ngata, Takahiro Ito, and Kazuhisa Sekimizu., Transcriptional repression of the IMD2 gene mediated by transcriptional co-activator Sub1
Genes to Cell. 13: 1113-1126 (2008)
3. Keiko Tano, Makiko Nagata, Nobuyoshi Akimitsu., S-II mediated gene regulation
Drug Discov Ther. 2(1):136-139 (2008)
4. Ryoko Iijima, Takekazu Kunieda, Shinji Yamaguchi, Hiroko Kamigaki, Ikuko Fujii-Taira, Kazuhisa Sekimizu, Takeo Kubo, Shunji Natori, and Koichi J.Homma., The Extracellular Adenosine Deaminase Growth Factor, ADGF/CECR1, Plays a Role in Xenopus Emryogenesis via the Adenosine/P1 Receptor
J. Biol. Chem. 283(4):2255-64 (2008)
5. Daisuke Morishita, Ryohei Katayama, Kazuhisa Sekimzu, Takashi Tsuruo, and Naoya Fujita., Pim Kinase Promote Cell Cycle Progression by Phosphorylating and Dwon-regulating p27Kip1 at the Transcriptional and Posttranscriptional Levels
Cancer Res. 68(13):5076-85 (2008)
6. Hamamoto H, Akimitsu N, Arimitsu N, Sekimizu K., Roles of the Duffy antigen and glycophorin A in malaria infection and erythrocyte
Drug Discov Ther. 2(2):58-63 (2008)
7. Ishii K, Hamamoto H, Sasaki T, Ikegaya Y, Yamatsugu K, Kanai M, Shibasaki M, Sekimizu K., Pharmacologic Action of Oseltamivir on the Nervous System.
Drug Discov Ther. 2(1):24-34 (2008)
8. Kurokawa K, Nishida S, Ishibashi M, Mizumura H, Ueno K, Yutsudo T, Maki H, Murakami K, Sekimizu K., Staphylococcus aureus MurC participates in L-alanine recognition via Histidine 343, a conserved motif in the shallow hydrophobic pocket.
J Biochem. 143(3):417-24 (2008)
9. Orihara Y, Hamamoto H, Kasuga H, Shimada T, Kawaguchi Y, Sekimizu K., A silkworm-baculovirus model for assessing the therapeutic effects of anti-viral compounds: characterization and application to the isolation of anti-virals from traditional medicines.
J. Gen. Virol. 89(1):188-194 (2008)
10. Nagata M, Kaito C, Sekimizu K., Phosphodiesterase activity of CvfA is required for virulence in Staphylococcus aureus.
J. Biol. Chem. 283(4):2176-2184
11. Ishii K, Hamamoto H, Kamimura M, Sekimizu K., Activation of the silkworm cytokine by bacterial and fungal cell wall components via a reactive oxygen species-triggered mechanism.
J. Biol. Chem. 283(4):2185-2191
12. Koyama H, Sumiya E, Ito T, Sekimizu K., Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae.
FEMS Yeast Research. 8(2):193-194
II. 著書
1. 生物薬科学実験講座 1.「タンパク質」関水和久・富田基郎著
廣川書店 平成20年4月20日初版発行
2007
I. 原著論文
1. Takabatake N, Okamura M, Yokoyama N, Ikehara Y, Akimitsu N, Arimitsu N, Hamamoto H, Sekimizu K, Suzuki H, Igarashi I., Glycophorin A-knockout mice, which lost sialoglycoproteins from the red blood cell membrane, are resistant to lethal infection of Babesia rodhaini.
Vet Parasitol. 148(2):93-101 (2007)
2. Kaito C and Sekimizu K., A silkworm model of pathogenic bacterial infection.
Drug Discov Ther. 1(2):89-93 (2007)
3. Ishii K, Hamamoto H, Sekimizu K., A novel method to suppress the dispersal of Japanese cedar pollen by inducing morphologic changes with weak alkaline solutions.
Drug DiscovTher. 1(2):124-129 (2007)
4. Ito T, Saso K, Arimitsu N, Sekimizu K., Defective FESTA/EAF2-mediated transcriptional activation in S-II-deficient embryonic stem cells.
Biochem. Biophys. Res. Commun. 363(3):603-609 (2007)
5. Ito T, Doi K, Matsumoto N, Kakihara F, Noiri E, Hasegawa S, Tokunaga K, Sekimizu K., Lack of polymorphisms in the coding region of the highly conserved gene encoding transcription elongation factor S-II (TCEA1).
Drug Discov Ther. 1(1): 9-11 (2007)
6. Ishino T, Kunieda T, Natori S, Sekimizu K, Kubo T., Identification of novel members of the Xenopus Ca2+ -dependent lectin family and analysis of their gene expression during tail regeneration anddevelopment.
J. Biochem. (Tokyo) 141(4):479-488 (2007)
7. Hattori K, Hirayama M, Suzuki H, Hamamoto H, Sekimizu K, Tamura HO., Cloning and expression of a novel sulfotransferase with unique substrate specificity from Bombyx mori.
Biosci. Biotechnol. Biochem. 71(4):1044-1051 (2007)
8. Ashibe B, Hirai T, Higashi K, Sekimizu K, Motojima K., Dual subcellular localization in the endoplasmic reticulum and peroxisomes and a vital role in protecting against oxidative stress of fatty aldehyde dehydrogenase are achieved by alternative splicing.
J. Biol. Chem. 282(28):20763-20773 (2007)
9. Li, Y., Kurokawa, K., Reutimann, L., Mizumura, H., Matsuo, M., and Sekimizu, K., dnaB and dnaI temperature-sensitive mutants of Staphylococcus aureus: evidence for involvement of DnaB and DnaI in synchrony regulation of chromosome replication.
Microbiol. 153 (10): 3370-3379 (2007)
10. Ishibashi, M., Kurokawa, K., Nishida, S., Ueno, K., Matsuo, M., and Sekimizu, K., Isolation of temperature-sensitive mutations in murC of Staphylococcus aureus.
FEMS Microbiol. Lett. 274(2):204-209 (2007)
11. Ikeda, R., Saito, F., Matsuo, M., Kurokawa, K., Sekimizu, K., Yamaguchi, M., and Kawamoto, S., The contribution of the mannan backbone of cryptococcal glucuronoxylomannan and a glycolytic enzyme of Staphylococcus aureus to contact mediated killing of Cryptococcus neoformans.
J. Bacteriol. 189:4815-4826 (2007)
12. Kurokawa, K., Kaito, C., Sekimizu, K., Two-Component Signaling in Virulence of S. aureus: Silkworm Larvae-Pathogenic Agents Infection Model for Virulence.
Methods Enzymol. 422:233-244 (2007)
13. Koyama, H., Ito, T., Nakanishi, T., Sekimizu, K., Stimulation RNA polymerase II transcript cleavage activity contributes to maintain transcriptional fidelity in yeast.
Genes to Cells. 12 (5):547-559. (2007)
14. Matsumoto Y, Kaito C, Morishita D, Kurokawa K, Sekimizu K., Regulation of exoprotein gene expression by the Staphylococcus aures cvfB gene.
Infect. Immun. 75 (4):1964-1972 (2007)
15. Nagata M, Arimitsu N, Ito T, Sekimizu K., Antioxidant N-acetyl-l-cysteine inhibits erythropoietin-induced differentiation of erythroid progenitors derived from mouse fetal liver.
Cell Biol Int. 31 (3):252-256 (2007)
16. Kaito C, Sekimizu K., Colony Spreading in Staphylococcus aureus.
J Bacteriol. 189 (6):2553-2557 (2007)
2006
I. 原著論文
1. Kaito C, Morishita D, Matsumoto Y, Kurokawa K, Sekimizu K., Novel DNA binding protein SarZ contributes to virulence in Staphylococcus aureus.
Mol Microbiol. 62 (6):1601-1617 (2006)
2. Hossain MS, Hamamoto H, Matsumoto Y, Razanajatovo IM, Larranaga J, Kaito C, Kasuga H, Sekimizu K., Use of silkworm larvae to study pathogenic bacterial toxins.
J Biochem (Tokyo). 140(3):439-444. (2006)
3. Ito T, Arimitsu N, Takeuchi M, Kawamura N, Nagata M, Saso K, Akimitsu N, Hamamoto H, Natori S, Miyajima A, Sekimizu K., Transcription elongation factor S-II is required for definitive hematopoiesis.
Mol Cell Biol. 26(8):3194-203. (2006)
4. Nishida, S., Kurokawa, K., Matsuo, M., Sakamoto, K., Ueno, K., Kita, K., and Sekimizu, K., Identification and characterization of amino acid residues essential for the active site of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Staphylococcus aureus.
J. Biol. Chem. 281(3):1714-24. (2006)
5. Eguchi Y, Ueno S, Kaito C, Sekimizu K, Shiokawa K., Cleavage and survival of Xenopus embryos exposed to 8 T static magnetic fields in a rotating clinostat.
Bioelectromagnetics. 27(4):307-313. (2006)
6. Murai M, Kurokawa K, Ichihashi N, Matsuo M, and Sekimizu K. Isolation of a temperature-sensitive dnaA mutant of Staphylococcus aureus.
FEMS Microbiol Lett. 254(1):19-26. (2006)
II. 著書（総説）
Hiroshi Hamamoto, Jorge Larranaga, Masae Sekine, Takemitsu Furuchi, Masumi Katane, Hiroshi Homma, Norio Matsuki and Kazuhisa Sekimizu. D-GLUTAMIC ACID INDUCED MUSCLE CONTRACTION IN SILKWORM LARVA, In:D-Amino Acids: A new Frontier in Amino Acid and Protein Resarch.
2005
I. 原著論文
1. Rungruang T, Kaneko O, Murakami Y, Tsuboi T, Hamamoto H, Akimitsu N, Sekimizu K, Kinoshita T and Torii M., Erythrocyte surface glycosylphosphatidyl inositol anchored receptor for the malaria parasite.
Mol Biochem Parasitol. 140, 13-21. (2005)
2. Aramaki Y, Ogawa K, Toh Y, Ito T, Akimitsu N, Hamamoto H, Sekimizu K, Matsusue K, Kono A, Iguchi H and Takiguchi S., Direct interaction between metastasis-associated protein 1 and endophilin 3.
FEBS Lett. 579, 3731-3736. (2005)
3. Hamamoto H, Kamura K, Razanajatovo I M, Murakami K, Santa T, Sekimizu K. Effects of molecular mass and hydrophobicity on transport rates through non-specific pathways of the silkworm larva midgut.
Int J Antimicrob Agents. 26, 38-42. (2005)
4. Kawamura N, Kurokawa K, Ito T, Hamamoto H, Koyama H, Kaito C, and Sekimizu K. Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation.
Genes Cells. 10, 477-487. (2005)
5. Kaito C, Kurokawa K, Matsumoto Y, Terao Y, Kawabata S, Hamada S and Sekimizu K. Silkworm-pathogenic bacteria infection model for identification of novel virulence genes.
Mol Microbiol. 56, 34-44. (2005)
カイコ幼虫の感染モデルを用いて、３つの黄色ブドウ球菌の病原性遺伝子を発見した。
6. Sekimizu K, Larranaga J, Hamamoto H, Sekine M, Furuchi T, Katane M, Homma H and Matsuki N. D-Glutamic acid-induced muscle contraction in the silkworm, Bombyx mori.
J Biochem. 137, 199-203. (2005)
Dグルタミン酸がカイコ幼虫の筋肉収縮を引き起こす神経伝達物質であることを発見した。
7. Hamamoto H and Sekimizu K. Evaluation of the therapeutic effects of antibiotics using silkworm as an animal model.
Res Adv Antimicrob Agents Chemother. 5, 1-23. (2005)
8. Shiokawa K, Takayama E, Higo T, Kuroyanagi S, Kaito C, Hara H, Kajitani M, Sekimizu K, Tadakuma T, Miura K, Igarashi K, Yaoita Y. Occurrence of pre-MBT synthesis of caspase-8 mRNA and activation of caspase-8 prior to execution of SAMDC (S-adenosylmethionine decarboxylase)-induced, but not p53-induced, apoptosis in Xenopus late blastulae.
Biochem and Biophys Res Commun. 336 682-691. (2005)
9. Yamaguchi S, Katagiri S, Sekimizu K, Natori S, Homma KJ., Involvement of EDTP, an Egg-Derived Tyrosine Phosphatase, in the Early Development of Drosophila melanogaster.
J Biochem (Tokyo). 138, 721-728. (2005)
2004
I. 原著論文
1. Hossain MS, Kurokawa K, Sekimizu K. Induction of fusion-competent myoblast-specific gene expression during myogenic differentiation of Drosophila Schneider cells by DNA double-strand breaks or replication inhibition.
BBA. 1743:176-86. (2004)
DNAの二重鎖を切断する薬剤により、ショウジョウバエの培養細胞を筋肉に分化させることができることを発見した。
2. Nakata A, Ito T, Nagata M, Hori S, Sekimizu K. GRIP1tau, a novel PDZ domain-containing transcriptional activator, cooperates with the testis-specific transcription elongation factor SII-T1.
Genes Cells. 9:1125-35. (2004)
精巣特異的に発現する転写伸長因子SII-T1と協調的に働く転写制御タンパク質を発見した。
3. Hossain MS, Kurokawa K, Akimitsu N, Sekimizu K. DNA topoisomerase II is required for the G0-to-S phase transition in Drosophila Schneider cells, but not in yeast.
Genes Cells. 9:905-17. (2004)
DNAトポイソメラーゼIIが、ショウジョウバエの培養細胞ではG0期からS期への以降に必要であるが、酵母細胞では不必要であることを示した。
4. Ishida T, Akimitsu N, Kashioka T, Hatano M, Kubota T, Ogata Y, Sekimizu K, Katayama T. DiaA, a novel DnaA-binding protein, ensures the timely initiation of Escherichia coli chromosome replication.
J Biol Chem. 279:45546-55. (2004)
大腸菌のDNA複製開始タンパク質DnaAに結合してDNA複製開始を制御するタンパク質DiaAを発見した。
5. Akimitsu N, Kim HS, Hamamoto H, Kamura K, Fukuma N, Arimitsu N, Ono K, Wataya Y, Torii M, Sekimizu K. Duffy antigen is important for the lethal effect of the lethal strain of Plasmodium yoelii 17XL.
Parasitol Res. 93:499-503. (2004)
赤血球膜状のダフィー抗原と呼ばれるタンパク質が、マラリア原虫の病原性発揮に関わっていることを発見した。
6. M.Saito, T.Santa, M.Tsunoda, H.Hamamoto, and N.Usui, An automated analyzer for vancomycin in plasma samples by column-switching high-performance liquid chromatography with UV detection.
Biomed Chromatogr. 18:735-38. (2004)
血漿中のバンコマイシン濃度を感度よく自動的に定量する方法を開発した。
7. Jilma B, Akimitsu N, Fukuma N, Sekimizu K, Jilma-Stohlawetz P. Man, mouse and Duffy genotype-phenotype-specific pharmacokinetics of monocyte chemotactic protein-1.
Transfus Med. 14:251-52. (2004)
赤血球膜状のダフィー抗原がサイトカインの体内動態に影響を及ぼすことを発見した。
8. Okuyama Y, Cho JH, Nakajima Y, Homma K, Sekimizu K, Natori S. Binding between azurocidin and calreticulin: its involvement in the activation of peripheral monocytes.
J Biochem (Tokyo). 135:171-77. (2004)
アズロシジンとカルレティキュリンというタンパク質の相互作用により、白血球の活性化が引き起こされることを発見した。
9. Li Y, Kurokawa K, Matsuo M, Fukuhara N, Murakami K, Sekimizu K. Identification of temperature-sensitive dnaD mutants of Staphylococcus aureus that are defective in chromosomal DNA replication.
Mol Genet Genomics. 271:447-57. (2004)
黄色ブドウ球菌dnaDと呼ばれる遺伝子の産物がDNA複製に必要であることを、温度感受性変異株を分離することにより初めて示した。
10. Hamamoto H, Kurokawa K, Kaito C, Kamura K, Manitra Razanajatovo I, Kusuhara H, Santa T, Sekimizu K. Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms.
Antimicrob Agents Chemother. 48:774-79. (2004)
カイコ幼虫を使って、抗真菌剤・抗菌剤の治療効果を定量的に評価できることを述べた。ED50値は、マウスなどの哺乳類で得られる値と一致していた。カイコ幼虫は、病態モデル動物として今後重要性を増すと期待される。この論文は、2004年3月のアメリカ細菌学会誌11誌の掲載論文の中からベスト６に選ばれた。
11. Oku Y, Kurokawa K, Ichihashi N, Sekimizu K. Characterization of the Staphylococcus aureus mprF gene, involved in lysinylation of phosphatidylglycerol.
Microbiology. 150:45-51. (2004)
黄色ブドウ球菌のmprF遺伝子は、リジルフォスファチジルグリセロール合成酵素をコードしていることを示した。
12. Takeuchi K, Takahashi H, Sugai M, Iwai H, Kohno T, Sekimizu K, Natori S, Shimada I. Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR.
J Biol Chem. 279:4981-87. (2004)
ハエの幼虫の体液中に見いだされたザーペシンと呼ばれる抗菌ペプチドが複合体を形成して細菌の膜に穴をあけることをNMRによって示した。
II. 著書（総説）
1. 浜本洋、関水和久, カイコ幼虫をモデル動物とした抗生物質の治療効果の評価
医学のあゆみ 210:1011-1012. (2004)
カイコ幼虫が、抗生物質の治療評価を行う上に有用であることを示した。