Rapid determination of amino acids in biological samples using a monolithic silica column
Y. Song, T. Funatsu, and M. Tsunoda
Amino Acids, in press
Amino acids analysis using in loop derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole
M. Tsunoda, Y. Song, T. Funatsu
Current Analytical Chemistry - Special Issue on Analytical Derivatization, in press
Initiation factor 2 and 50S cooperate to lock mRNAs on the ribosome during initiation
T. Masuda, A. N. Petrov, R. Iizuka, T. Funatsu, J. D. Puglisi, and S. Uemura
Proceedings of the National Academy of Sciences USA, in press
Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy
Okabe, K., N. Inada, C. Gota, Y. Harada, T. Funatsu, and S. Uchiyama
Nat. Commun. 3: 705 doi: 10.1038/ncomms1714 (2012)
Catecholamine analysis with strong cation exchange column liquid chromatography-peroxyoxalate chemiluminescence reaction detection
M. Tsunoda, and T. Funatsu
Anal. Bioanal. Chem. 402: 1393-1397 (2012)
Dynamic association-dissociation and harboring of endogenous mRNAs in stress granules
J. Zhang, K. Okabe, T. Tani, and T. Funatsu
J. Cell Sci. 124: 4087-4095 (2011)
Damage-free Fabrication of Perfluoropolymer Microaperture Array Device for Single-molecule Imaging
T. Ono, R. Iizuka, T. Akagi, T. Funatsu, and T. Ichiki
Trans. Mater. Res. Soc. Jpn. 36: 553-556 (2011)
Real-time single-molecule observation of green fluorescent protein synthesis by immobilized ribosomes
R. Iizuka, T. Funatsu, and S. Uemura
Methods Mol Biol. 778, 215-228 (2011)
Single-Molecule Fluorescence Polarization Study of Conformational Change in Archaeal Group II Chaperonin
R. Iizuka, T. Ueno, N. Morone, and T. Funatsu
PLoS ONE 6, e22253 (2011)
Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition
M. Haneoka, Y. Shirasaki, H. Sugino, T. Aoki, T. Arakawa, K. Ozaki, D. H. Yoon, N. Ishii, R. Iizuka, S. Shoji, and T. Funatsu
Sensor Actuat. B-Chem. 159, 314-320 (2011)
p38 MAP kinase regulates the expression of genes in the dopamine sysnthesis pathway through phosphorylation of NR4A nuclear receptors.
Y. Sekine, S. Takagahara, R. Hatanaka, T. Watanabe, H. Oguchi, T. Noguchi, I. Naguro, K. Kobayashi, M. Tsunoda, T. Funatsu, H. Nomura, T. Toyoda, N. Matsuki, E. Kuranaga, M. Miura, K. Takeda, and H. Ichijo
J. Cell Science In press
Biocompatible, surface functionalized mesoporous titania nanoparticles for intracellular imaging and anticancer drug delivery
K. Wu, K. C., Y. Yamauchi, C. Hong, Y. Yang, Y. Liang, T. Funatsu, and M. Tsunoda
Chem. Commun. 47: 5232-5234 (2011)
Fabrication of zero-mode waveguide by ultraviolet nanoimprint lithography lift-off process
J. Wada, S. Ryu, Y. Asano, T. Ueno, T. Funatsu, T. Yukawa, J. Mizuno, and T. Tanii
Jpn. J. Appl. Phys. 50: 06GK07 (2011)
Kinetic study of de novo chromophore maturation of fluorescent proteins
R. Iizuka, M. Yamagishi-Shirasaki, and T. Funatsu
Anal. Biochem. 414:173-178 (2011)
Extraction of catecholamines from urine using a monolithic silica disk-packed spin column and high-performance liquid chromatography-electrochemical detection
M. Tsunoda, C. Aoyama, S. Ota, T. Tamura, and T. Funatsu
Analytical Methods. 3: 582-585 (2011)
Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2’O-methyl RNA probes in living cells
K. Okabe, Y. Harada, J. Zhang, H. Tadakuma, T. Tani, and T. Funatsu
Nucleic Acids Res. 34: e20 (2011)
Amino acids analysis using a monolithic silica column after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F)
Y. Song, T. Funatsu, M. Tsunoda
J. Chromatogr. B, 879: 335-340 (2011)
Microfluidic cell sorter with flow switching triggered by a sol-gel transition of a thermo-reversible gelation polymer
K. Ozaki, H. Sugino, Y. Shirasaki, T. Aoki, T. Arakawa, T. Funatsu and S. Shoji
Sensor Actuat. B-Chem.150: 449-455 (2010)
Integration in a multilayer microfluidic chip of 8 parallel cell sorters with flow control by sol-gel transition of thermoreversible gelation polymer
H. Sugino, T. Arakawa, Y. Nara, Y. Shirasaki, K. Ozaki, S. Shoji and T. Funatsu
Lab Chip, 10: 2559-2565 (2010)
Single-molecule study on the decay process of the football-shaped GroEL-GroES complex using zero-mode waveguides
T. Sameshima, R. Iizuka, T. Ueno,J. Wada, M. Aoki, N. Shimamoto, I. Ohdomari, T. Tanii, and T. Funatsu
J. Biol. Chem. 285: 23159-23164 (2010)
Denatured proteins facilitate the formation of the football-shaped GroEL-GroES complex
T. Sameshima, R. Iizuka, T. Ueno, T. Funatsu
Biochem. J. 427: 247-254 (2010)
Hyperactivity in novel environment with increased dopamine and impaired novelty preference in apoptosis signal-regulating kinase 1 (ASK1)-deficient mice
Kumakura, K., H. Nomura, T. Toyoda, K. Hashikawa, T. Noguchi, K. Takeda, H. Ichijo, M. Tsunoda, T. Funatsu, D. Ikegami, M. Narita, T. Suzuki and N. Matsuki
Neuroscience Research, 66: 313-320 (2010)
Use of Folded Micromachined Pillar Array Column with Low-Dispersion Turns for Pressure-Driven Liquid Chromatography
C. Aoyama, A. Saeki, M. Noguchi, Y. Shirasaki, S. Shoji, T. Funatsu, J. Mizuno, and M. Tsunoda
Anal. Chem. 82: 1420-1426 (2010)
Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography
Tsunoda M., C. Aoyama, H. Nomura, T. Toyoda, N. Matsuki, and T. Funatsu
J. Pharm. Biomed. Anal. 51: 712-715 (2010)
Size-dependent accumulation of mRNA at the leading edge of chicken embryo fibroblasts
Yamagishi M., Y. Shirasaki, and T. Funatsu
Biochem. Biophys. Res. Commun. 390: 750-754 (2009)
cDNA display: A novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions
Yamaguchi J., M. Naimuddin, M. Biyani, T. Sasaki, M. Machida, T. Kubo, T. Funatsu, Y. Husimi, and N. Nemoto
Nucleic Acids Res.37: e108 (2009)
Single-molecule imaging of β-actin mRNAs in the cytoplasm of a living cell
Yamagishi M., Y. Ishihama, Y. Shirasaki, H. Kurama, and T. Funatsu
Exp. Cell Res. 315: 1142-1147 (2009)
Single molecule tracking of quantum dot-labeled mRNAs in a cell nucleus
Ishihama Y., and T. Funatsu
Biochem. Biophys. Res. Commun. 381: 33-38 (2009)
On-chip mirofluidic sorting with fluorescence spectrum detection and multiway separation
Sugino H., K. Ozaki, Y. Shirasaki, T. Arakawa, S. Shoji, and T. Funatsu
Lab Chip 9: 1254-1260 (2009)
Hydrophilic fluorescent nanogel thermometer for intracellular thermometry
Gota C., K. Okabe, T. Funatsu, Y. Harada, and S. Uchiyama
J. Am. Chem. Soc. 131: 2766-2767 (2009)
Infrared laser-mediated gene induction in targeted single cells in vivo
Kamei, Y., M. Suzuki, K. Watanabe, K. Fujimori, T. Kawasaki, T. Deguchi, Y. Yoneda, T. Todo, S. Takagi, T. Funatsu, and S. Yuba
Nat. Methods 6: 79-81 (2009)
Determination of selenomethionine by high-performance liquid chromatography-fluorescence detectioncoupled with on-line oxidation
Aoyama C., M. Tsunoda, T. Funatsu
Anal. Sci. 25: 63-65 (2009)
Kinetic analysis of conformational changes of GroEL based on the fluorescence of tyrosine 506
Hosono, K., T. Ueno, H. Taguchi, F. Motojima, T. Zako, M. Yoshida, and T. Funatsu
Protein J. 27: 461-468 (2008)
Effect of the C-terminal truncation on the functional cycle of chaperonin GroEL: Implication that the C-terminal region facilitates the transition from the folding-arrested to the folding-competent state
Suzuki, M., T. Ueno, R. Iizuka, T. Miura, T. Zako, R. Akahori, T. Miyake, N. Shimamoto, M. Aoki, T. Tanii, I. Ohdomari, and T. Funatsu
J. Biol. Chem. 283: 23931-23939 (2008)
Football- and bullet-shaped GroEL-GroES complexes coexist during the reaction cycle
Sameshima, T., T. Ueno, R. Iizuka, N. Ishii, N. Terada, K. Okabe, and T. Funatsu
J. Biol. Chem. 283: 23765-23773 (2008)
Visualization of the molecular dynamics of LPS on the plasma membrane of murine macrophages by TIRFM
Shawkat, S., R. Karima, T. Tojo, H. Tadakuma, S. Saitoh, S. Akashi-Takamura, K. Miyake, T. Funatsu, and K.Matsushima
J. Biol. Chem. 283: 22962-22971 (2008)
Real-Time Imaging of Single-Molecule Fluorescence with a Zero-Mode Waveguide for the Analysis of Protein-Protein Interaction
Miyake, T., T. Tanii, H. Sonobe, R. Akahori, N. Shimamoto, T. Ueno, T. Funatsu, and I. Ohdomari
Anal. Chem. 80: 6018-6022 (2008)
Single molecule imaging of full protein synthesis by immobilized ribosomes
Uemura, S., R. Iizuka, T. Ueno, Y. Shimizu, H. Taguchi, T. Ueda, J.D. Puglisi, and T. Funatsu
Nucleic Acids Res. 36: e70 (2008)
Oxidative stress increase 6-nitronorepinephrine and 6-nitroepinephrine concentrations in rat brain
Tsunoda, M., E. Uchino, K. Imai, and T. Funatsu
Biomed. Chromatogr. 22: 572-574 (2008)
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin
Sakamoto, A., M. Yamagishi, T. Watanabe, Y. Aizawa, T. Kato, and T. Funatsu
J. Biosci. Bioeng. 105: 238-242 (2008)
The dynamics of pre-mRNAs and poly(A)+ RNA at speckles in living cells revealed by iFRAP studies
Ishihama, Y., H. Tadakuma, T. Tani, and T. Funatsu
Exp. Cell Res. 314: 748-762 (2008)
Rapid multi-reagents exchange TIRFM microfluidic system for a single biomolecular imaging
Arakawa T., T. Samesima, Y. Sato, T. Ueno, Y. Shirasaki, T. Funatsu, and S. Shoji
Sensors and Actuators B: Chemical, 128:218-225 (2007)
Measurement of Catechol-O-methyltransferase Activity in the Brain of Dahl Salt-Sensitive Rats.
Hirano Y., M. Tsunoda, T. Shimosawa, T. Fujita, T. Funatsu
Biol Pharm Bull. 30 (11): 2178-2180 (2007)
Determination of nitrocatecholamines in rat brain using high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection
Tsunoda, M. E. Uchino, K. Imai, K. Hayakawa, and T. Funatsu
J. Chromatogr. A. 1164: 162-166 (2007)
Fluorescence millisecond oscillation in polar solvents regulates fluorescence intensity of colloidal quantum dots’ solution
Hoshino, A., K. Omata, S. Takami, T. Adschiri, N. Terada, T. Funatsu, M. Yasuhara, and K. Yamamoto
J. Nanophoton. 1: 013516 (2007)
Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template
Miyake T., T. Tanii, K. Kato, T. Zako, T. Funatsu, and I. Ohdomari
Nanotechnology. 18: 305304 (6pp) (2007)
On-chip Cell Sorting System Using Thermoreversible Gelation Polymer
Shirasaki, Y., H. Sugino, M. Tatsuoka, J. Mizuno, S. Shoji, and T. Funatsu
IEEE J. Sel. Top. Quant. Electron., 13: 223-227 (2007)
Suppression of COMT activity through blunting a2-adrenoceptor can explain hypertension in Dahl Salt-Sensitive rats.
Hirano, Y., M. Tsunoda, T. Shimosawa, H. Matsui, T. Fujita, T. Funatsu
Hypertens. Res., 30: 269-78 (2007)
Determination of potent antisense oligonucleotides in vitro by semi-empirical rules
Yanagihara, N. H. Tadakuma, Y. Ishihama, K. Okabe, T. Funatsu
J. Biosci. Bioeng. 103: 270-277 (2007)
Size distribution of linear and helical polymers in actin solution analyzed by photon counting histogram
Terada, N. T. Shimozawa, S. Ishiwata, and T. Funatsu
Biophys. J. 92: 2162-2171 (2007)
Three-dimensional sheath flow sorting microsystem using thermosensitive hydrogel.
Arakawa, T. Y. Shirasaki, T. Aoki, T. Funatsu, and S. Shoji
Sensors and Actuators A: Physical, 135: 99-105 (2007)
Label-free DNA sensors using ultrasensitive diamond field-effect transistors in solution.
Song K.S., G.J. Zhang, Y. Nakamura, K. Furukawa, T. Hiraki, J.H. Yang, T. Funatsu, I. Ohdomari and H. Kawarada
Phys Rev E Stat Nonlin Soft Matter Phys. 74: 041919.
High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel
Arakawa, T. Y. Shirasaki, T. Izumi, T. Aoki, H. Sugino, T. Funatsu, and S. Shoji
Meas. Sci. Techn. 17: 3141-3146 (2006)
Localization of Prefoldin Interaction Sites in the Hyperthermophilic Group II Chaperonin and Correlations between Binding Rate and Protein Transfer Rate.
Zako, T., Y. Murase, R. Iizuka, T. Yoshida, T. Kanzaki, N. Ide, M. Maeda, T. Funatsu, and M. Yohda
J. Mol. Biol. 364: 110-120 (2006).
Cellular responses of the ciliate, Tetrahymena thermophila, to far infrared irradiation.
Shiurba, R. T. Hirabayashi, M. Masuda, A. Kawamura, Y. Komoike, W. Klitz, K. Kinowaki, T. Funatsu, S. Kondo, S. Kiyokawa, T. Sugai, K. Kawamura, H. Namiki, and T. Higashinakagawa
Photochem. Photobiol. Sci. 5: 799-807 (2006).
Determination of NG,NG-dimethyl-l-arginine in rat plasma and dimethylarginine dimethylaminohydrolase activity in rat kidney using a monolithic silica column.
Nonaka S, M. Tsunoda C, Aoyama and T. Funatsu
J Chromatogr B, 843: 170-174 (2006)
Suppression of thiol exchange reaction in the determination of reduced-form thiols by high-performance liquid chromatography with fluorescence detection after derivatization with fluorogenic benzofurazan reagent, 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate and 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole.
Santa T, C. Aoyama, T. Fukushima, K. Imai, and T. Funatsu
Biomed Chromatogr. 20(6-7):656-61 (2006).
Imaging of single mRNA molecules moving within a living cell nucleus
Tadakuma, H., Y. Ishihama, T. Shibuya, T. Tani, and T. Funatsu
Biochem Biophys Res Commun. 344: 772-779 (2006)
A further study on the combined use of internal standard and isotope-labeled derivatization reagent for expansion of linear dynamic ranges in liquid chromatography-electrospray mass spectrometry.
Tsukamoto, Y., T. Santa, H. Yoshida, H. Miyano, T. Fukushima, K. Hirayama, K. Imai, and T. Funatsu
Biomed. Chromatogr.20: 1049-1055 (2006)
DNA micropatterning on polycrystalline diamond via one-step direct amination
Zhang, G.J., K.S. Song, Y. Nakamura, T. Ueno, T. Funatsu, I. Ohdomari, H. Kawarada
Langmuir 22: 3728-3734 (2006)
Catecholamine analysis with microcolumn LC-peroxyoxalate chemiluminescence reaction detection
Tsunoda, M., M. Nagayama, T. Funatsu, S. Hosoda, and K. Imai.
Clin. Chim. Acta. 366: 168-173 (2006)
Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells: Nuclear mRNA export is coupled to ongoing gene transcription
Tokunaga, K., T. Shibuya, Y. Ishihama, H. Tadakuma, M. Ide, M. Yoshida, T. Funatsu, Y. Ohshima, and T. Tani.
Genes to Cells. 11: 305-317 (2006)
On-chip cell sorting system using laser-induced heating of a thermo-reversible gelation polymer to control flow.
Shirasaki, Y., J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, and T. Funatsu.
Anal. Chem. 78: 695-701 (2006)
Synthesis of the isotope-labeled derivatization reagent for carboxylic acids, 7-(N,N-dimethylaminosulfonyl)-4 -(aminoethyl)piperazino-2,1,3-benzoxadiazole (d6) (DBD-PZ-NH2 (D)), and its application to the quantification and the determination of relative amount of fatty acids in rat plasma samples by high-performance liquid chromatography/mass spectrometry.
Tsukamoto Y, T. Santa, H. Yoshida, H. Miyano, T. Fukushima, K. Hirayama, K. Imai and T. Funatsu
Biomedical Chromatography 20: 358-364 (2006).
Analysis of Nuclear Microenvironments by Translational Diffusion of GFP Using Fluorescence Correlation Spectroscopy
Terada, N., H. Tadakuma, Y. Ishihama, M. Yamagishi, T. Zako, and T. Funatsu
Bioimages. 13: 1-10 (2005)
An improved assay for catechol-O-methyltransferase activity utilizing norepinephrine as an enzymatic substrate and reversed-phase high-performance liquid chromatography with fluorescence detection.
Aoyama, N., M. Tsunoda, and K. Imai.
J. Chromatogr. A, 1074: 47-51 (2005)..
Determination of methylated arginines by column-switching high-performance liquid chromatography-fluorescence detection.
Tsunoda, M., S. Nonaka, T. Funatsu.
Analyst, 130: 1410-1413 (2005)
Facilitated release of substrate protein from prefoldin by chaperonin.
Zako T., R. Iizuka, M. Okochi, T. Nomura, T. Ueno, H. Tadakuma1, M. Yohda and T. Funatsu.
FEBS Lett. 579: 3718-3724 (2005).
Nanoscale patterning of protein using electron beam lithography of organosilane self-assembled monolayers.
Zhang, G.-J., T. Tanii, T. Zako, T. Hosaka, T. Miyake, Y. Kanari, T. Funatsu, and I. Ohdomari.
Small, 1: 833-837. (2005)
Hybridization of deoxyribonucleic acid immobilization of green fluorescent protein on nanostructured porganosilane templates.
Tanii, T., T. Hosaka, T. Miyake, Y. Kanari, G.-J. Zhang, T. Funatsu, and I. Ohdomari.
Jpn. J. App. Phys. 44: 5851-5855 (2005).
Synthesis of benzofurazan derivatization regents for carboxylic and acids and its application to analysis of fatty acids in rat plasma by high-performance liquid chromatography/electrospray ionization mass spectrometry.
Tsukamoto Y., T. Santa, H. Saimaru, K. Imai, and T. Funatsu.
Biomed. Chromatogy. in press
Rapid assay for catechol-O-methyltransferase activity by high-performance liquid chromatography-fluorescence detection.
Hirano, Y., M. Tsunoda, T. Funatsu, and K. Imai
J. Chromatogr. B, 819: 41-44 (2005)
High-performance liquid chromatographic assay of NG-monomethyl-L-arginine, NG,NG-dimethyl-L-arginine, and NG,NG'-dimethyl-L-arginine using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent reagent.
Nonaka, S., M. Tsunoda, K. Imai, and T. Funatsu
J. Chromatogr. A, 1066: 41-45 (2005)
Micropatterning oligonucletides on single crystal diamond surface by photolithography.
Zhang, G. H. Umezawa, H. Hata, T. Zako, T. Funatsu, I. Ohdomari, and H. Kawarada
Jpn. J. Appl. Phys. 44: L295-298 (2005)
Kinetic analysis of interactions between archaeal prefoldin and chaperonin.
Zako T., T.Funatsu, and M．Youhda
Recent Res. Develop. Biophys. 3: 475-483 (2004)
Preferential immobilization of biomolecules on silicon microstructure array by means of electron beam lithography on organosilane self-assembled monolayer resist.
Tanii, T., T. Hosaka, T. Miyake, G. Zhang, T. Zako, T. Funatsu, and I. Ohdomari.
Appl.Surf.Sci. 234: 102-106 (2004)
Kinetics and binding sites for interaction of prefoldin with group II chaperonin: contiguous non-native substrate and chaperonin binding sites in archaeal prefoldin.
Okochi, M., T. Nomura, T. Zako, R. Iizuka, H. Ueda, T. Funatsu, M. Leroux, and M. Yohda:
J. Biol. Chem. 279: 31788-31795 (2004)
GroEL mediates protein folding with a two successive timer mechanism.
Ueno, T., T., H. Taguchi, H. Tadakuma, M. Yoshida, and T. Funatsu:
Mol. Cell 14: 423-434 (2004).
Patterning of DNA nanostructures on silicon surface by electron beam lithography of self-assembled monolayer.
Zhang, GJ., T. Tanii, T. Funatsu, and I. Ohdomari:
Chem Comm. 786-787 (2004)
The immobilization of DNA on microstructured patterns fabricated by maskless lithography.
Zhang, G., T. Tanii, T. Zako, T. Funatsu, and I. Ohdomari;
Sensors and Actuators B: 97: 243-248 (2004)
Rapid and Sensitive Detection Method of A Bacterium Using GFP Reporter Phage
Funatsu, T., T. Taniyama,T. Tajima, H. Tadakuma, and H. Namiki
Microbiol. Immuno. 46: 365-369.(2002)
Microscopicanalysis of polymerization dynamics with individual actin filaments
Fujiwara,, I., S.Takahashi, H.Tadakuma, T. Funatsu, and S.Ishiwata
Nature Cell Biol. 4: 666-673. (2002)
Rapid functional analysis of protein-protein interactions by fluorescent C-terminal labeling and single-molecule imaging
Yamaguchi, J.., N.Nemoto, T.Sasaki, A.Tokumasu, Y.Mimori-Kiyosue, T.Yagi, and T.Funatsu
FEBS Lett. 502: 79-83. (2001)
Single-molecule observation of protein-protein interactions in the chaperonin system
Taguchi, H., T. Ueno, H. Tadakuma, M. Yoshida, and T. Funatsu
Nature Biotechnol. 19: 861-865 (2001).
Imaging of Single Fluorescent Molecules Using Video-rate Confocal Microscopy
Tadakuma, H., J.Yamaguchi, Y. Ishihama, and T. Funatsu
Biochem Biophys Res Commun. 287: 323-327 (2001).
Spectral Fluctuation of a Single Fluorophore Conjugated to a Protein Molecule
Wazawa, T., Y. Ishii, T. Funatsu, and T. Yanagida
Biophys. J. 78: 1561-1569 (2000)
Single molecule imaging of RNA polymerase-DNA interactions in real time
Harada, Y., T. Funatsu, K. Murakami, Y. Nonoyama, A. Ishihama, and T. Yanagida
Biophys. J. 76: 709-715 (1999).
Fluorescence resonance energy transfer between single fluorophores attached to a coiled-coil protein in aqueous solution
Ishii, Y., T. Yoshida, T. Funatsu, T. Wazawa, and T. Yanagida
Chemical Physics. 247:163-173 (1999)
Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin
Ishijima, A., H. Kojima, T. Funatsu, M. Tokunaga, H. Higuchi, H. Tanaka, and T. Yanagida
Cell. 92: 161-171 (1998)..
Single molecular assay of the individual ATP turnovers by a myosin-GFP fusion protein expressed in vitro
Iwane, A.H., T. Funatsu, Y. Harada, M. Tokunaga, O. Ohara, S. Morimoto, and T. Yanagida
FEBS Letters. 407: 235-238.(1997)
Imaging and nano-manipulation of single bio-molecules
Funatsu, T., Y. Harada, H. Higuchi, M. Tokunaga, K. Saito, Y. Ishii, R. Vale, T. Yanagida
Biophys. Chem. 68: 63-72 (1997).
Inter-head distances in myosin attached to F-actin estimated by fluorescence energy transfer spectroscopy.
Ishiwata, S., M. Miki, I. Shin, T. Funatsu, K. Yasuda, and C.G. dosRemedios
Biophys. J. 73: 895-904 (1997)
Multiple- and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle: Unitary steps and forces
Ishijima,A., H. Kojima, H. Higuchi, H. Harada, T. Funatsu, and T. Yanagida
Biophys. J. 70: 383-400. (1996)
Direct observation of single kinesin molecules moving along microtubules
Vale, R.D., T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida
Nature. 380: 451-453 (1996).
Structural and functional reconstitution of thin filaments in the contractile apparatus of cardiac muscle
Fujita, H., K. Yasuda, S. Niitsu, T. Funatsu, and S. Ishiwata
Biophys. J. 71: 2307-2318. (1996)
Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
Funatsu,T., Y. Harada, M. Tokunaga, K. Saito, and T. Yanagida
Nature. 374: 555-559. (1995)
Single-molecule analysis of the actomyosin motor using nano-manipulation
Ishijima, A., Y. Harada, H. Kojima, T. Funatsu, H. Higuchi, and T. Yanagida
Biochem Biophys Res Commun 199: 1057-1063 (1994)
Structural and functional reconstitution of thin filaments in skeletal muscle
Funatsu, T., T. Anazawa, and S. Ishiwata
J. Muscle Res. Cell Motil. 15: 158-171. (1994)
Elastic filaments in situ in cardiac muscle: deep-etch replica analysis in combination with selective removal of actin and myosin filaments
Funatsu, T., E. Kono, H. Higuchi, S. Kimura, S. Ishiwata, T. Yoshioka, K. Maruyama, and S. Tsukita
J. Cell Biol. 120: 711-724 (1993).
Changes in mobility of chromaffin granules in actin network with its assembly and Ca2+-dependent disassembly by gelsolin
Miyamoto, S., T. Funatsu, S. Ishiwata, and S. Fujime
Biophys. J. 64: 1139-1149 (1993).
Time-resolved electron microscopic analysis of the behavior of myosin heads on actin filaments after photolysis of caged ATP
Funatsu, T., E. Kono, and S. Tsukita
J. Cell Biol. 121: 1053-1064 (1993)
Elastic filaments in skeletal muscle revealed by selective removal of thin filaments with plasma gelsolin
Funatsu, T., H. Higuchi, and S. Ishiwata
J. Cell Biol. 110: 53-62 (1990).