Dynamics of cell death signaling

Cell death is thought to be widely observed during development, tissue homeostasis and disease condition. However, reality is different. Even in the sensitive TUNEL assay, detection of apoptosis is not easy. Apoptotic cells are rapidly removed from body. We can only recognize the area massive cell death occurs. Live cell imaging using fluorescence resonance energy transfer (FRET) technology permits us to monitor cell-signaling activities simultaneously with cell behavior in real time. Live FRET imaging with a fast-scanning confocal microscope enable us to monitor in vivo dynamics of caspase activation. SCAT (sensor for caspase activation based on FRET) consists of ECFP and Venus, both of which are connected with a linker sequence containing cleavage sites of caspase. We succeeded in monitoring caspase-3 activation by SCAT3 in living
Drosophila pupa and mouse embryo.
We have generated FRET sensor for activated caspase-1, named SCAT1. We examined the spatial and temporal activation of caspase-1 in macrophages, and found that activation of caspase-1 was the manner of all-or-none (digital) at the single-cell level, with similar activation kinetics irrespective of the type of inflammasome or the intensity of the stimulus. Caspase-1 activated macrophage die immediately and real-time concurrent detection of caspase-1 activation and IL-1b release demonstrated that dead macrophages release a local burst of IL-1b in a digital manner. These dying macrophages as the main source of IL-1b within cell populations. These results highlight the value of single-cell analysis in understanding of the inflammasome system and chronic inflammatory diseases. Thus combination of live imaging and genetics revealed the active roles of caspase-mediated cell death during development and inflammation.



References
Liu, T., Yamaguchi, Y., Shirasaki, Y., Shikada, K., Yamagishi, M., Hoshino, K., Kaisho, T., Takemoto, K., Suzuki, T., Kuranaga, E., Ohara, O., and Miura, M.: Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response. Cell Rep 8, 974-982, 2014.
Yamaguchi, Y., Kuranaga, E., Nakajima, Y., Koto, A., Takemoto, K., and Miura, M.: In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe. Regulated Cell Death. ed. Ashkenazi, A.,  Wells , J., Yuan, J. Methods Enzymol. 544, 299-325, 2014
Yamaguchi, Y., Shinotsuka, N., Nonomura, K., Takemoto, K., Kuida, K., Yoshida, H., and Miura, M.: Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure. J. Cell Biol. 195, 1047-1060, 2011

Nakajima, Y-I, Kuranaga, E., Sugimura, K., Miyawaki, A., and Miura, M.: Non-autonomus apoptosis is triggered by local cell cycle progression during epithelial replacement in Drosophila. Mol. Cell Biol., 31 2499-2512, 2011 Koto, A., Kuranaga, E., and Miura, M.: Apoptosis ensures spacing pattern formation of Drosophila sensory organs. Current Biol.21, 278-287, 2011 Kuranaga, E., Matsunuma, T., Kanuka, H., Takemoto, K., Koto, A., Kimura, K and Miura, M.: Apoptosis controls the speed of looping morphogenesis in Drosophila male terminalia. Development138,1493-1499,2011


Back to Research page


---------------------------------------------------------------------------------------------------------------------
This page is administered by Department of Genetics All Rights Reserved, Copyright © 2015 Department of Genetics