Isao NAGURO

名黒 功 なぐろ いさお
略歴
平成 8年 3月 鳥取県立米子東高等学校 卒業
  平成12年 3月 東京大学 薬学部 卒業
  平成14年 3月 東京大学 大学院薬学系研究科 修士課程 修了
  平成17年 3月 東京大学 大学院薬学系研究科 博士課程 修了
  平成17年 4月 東京大学 大学院薬学系研究科 寄付講座教員
  平成18年 4月 東京大学 大学院薬学系研究科 助手
  平成19年 4月 東京大学 大学院薬学系研究科 助教
  平成25年 3月 東京大学 大学院薬学系研究科 講師
  平成28年12月 東京大学 大学院薬学系研究科 准教授
 免許・資格  
  平成12年 薬剤師免許取得
  平成24年 第1種放射線取扱主任者免許取得
CURRICULUM VITAE
Name:
 Isao Naguro
Date and place of birth: 6 March, 1978, Tottori Japan
Nationality: Japanese
Affiliation:  
  Position and title: Lecturer of The University of Tokyo
  Dept./ Laboratory: Laboratory of Cell Signaling ,Graduate School of Pharmaceutical Sciences
  Institution: The University of Tokyo
  Address: 7-3-1 Hongo, Bunkyo-ku, Tokyo
  Phone: +81-3-5841-4859
  Fax: +81-3-5841-4798
  E-mail: nagurois (at) mol.f.u-tokyo.ac.jp

Degrees:  
 
2005
Ph.D. in Pharmaceutical Science The University of Tokyo, Japan
 
2002
Masters in Pharmaceutical Science The University of Tokyo, Japan
 
2000
Bachelor of Science The University of Tokyo, Japan
Education and Academic Background:
  2013-present Lecturer, Laboratory of Cell Signaling, The University of Tokyo, Japan
   
    Subjects of Research:
  1. Analysis of the cellular signal transduction in response to extracellular stress (e.g. oxidative and osmotic stress), focusing on apoptosis signal-regulating kinase (ASK) family
  2. Establishment of the genome-wide siRNA screening system for mammalian cells using high-content image analyzers
  3. Single cell analysis of signal transduction relating to cell death
  2014 Jul   Cold Spring Harbor Laboratory course program (Single Cell Analysis)
  2010 Jul-Sep The visiting researcher of National University of Singapore, Department of Biological Sciences, Singapore
Purpose: Investigating the involvement of ASK3 in mechanical stress
Mentor: Dr. Sawada
  2005-2013 Assistant Professor, Laboratory of Cell Signaling, The University of Tokyo, Japan
  2002-2005 Ph.D. course in Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
    Thesis Title: “The regulation of Ca2+ signaling through voltage-dependent L-type Ca2+ cannel CaV1.2 and the interacting proteins”
  2000-2002 Master course in Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
  2000 BSc in Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Professional Societies:
  · The Japanese Biochemical Society
· The Molecular Biology Society of Japan
· The Japanese Pharmacological Society
· Japanese Cancer Association
· The Japanese Association for Molecular Target Therapy of Cancer
Publications:
  1. Hattori, K., Naguro, I., Okabe, K., Funatsu, T., Furutani, S., Takeda, K. and Ichijo, H.
    ASK1 signaling regulates brown and beige adipocyte function.
    Nat. Commun., 7: 11158 (2016).

  2. Maruyama, J., Kobayashi, Y., Umeda, T., Vandewalle, A., Takeda, K., Ichijo, H. and Naguro, I.
    Osmotic stress induces the phosphorylation of WNK4 Ser575 via the p38MAPK-MK pathway.
    Sci. Rep.
    , 6, 18710 (2016).

  3. Fujisawa, T., Takahashi, M., Tsukamoto, Y., Yamaguchi, N., Nakoji, M., Endo, M., Kodaira, H., Hayashi, Y., Nishitoh, H., Naguro, I., Homma, K. and Ichijo, H.
    The ASK1-specific inhibitors K811 and K812 prolong survival in a mouse model of amyotrophic lateral sclerosis.
    Hum. Mol. Genet., 25, 245-253 (2016).

  4. Fujisawa, T., Yamaguchi, N., Kadowaki, H., Tsukamoto, Y., Tsuburaya, N., Tsubota, A., Takahashi, H., Naguro, I., Takahashi, Y., Goto, J., Tsuji, S., Nishitoh, H., Homma, K. and Ichijo, H. A systematic immunoprecipitation approach reinforces the concept of common conformational alterations in amyotrophic lateral sclerosis-linked SOD1 mutants.
    Neurobiol. Dis., 82, 478-486 (2015).

  5. Kamiyama, M., Naguro, I., Ichijo, H.
    In vivo gene manipulation reveals the impact of stress-responsive MAPK pathways on tumor progression.
    Cancer Sci., (review article), 106, 785-796 (2015).


  6. Watanabe, T., Sekine, S., Naguro, I., Sekine, Y. and Ichijo, H.
    Apoptosis Signal-regulating Kinase 1(ASK1)-p38 pathway-dependent cytoplasmic translocation of the orphan nuclear receptor NR4A2 is required for oxidative stress-induced necrosis.
    J. Biol. Chem., 290, 10791-10803 (2015).


  7. Mosallanejad, K., Sekine, Y., Ishikura-Kinoshita, S., Kumagai, K., Nagano, T., Matsuzawa, A., Takeda, K., Naguro, I. and Ichijo, H.
    The DEAH-Box RNA Helicase DHX15 activates NF-κB and MAPK signaling downstream of MAVS during antiviral responses.
    Sci. Signal., 7, ra40 (2014).


  8. Maruyama, T., Araki, T., Kawarazaki, Y., Naguro, I., Heynen, S., Aza-Blanc, P., Ronai, Z., Matsuzawa, A. and Ichijo, H. 
    Roquin-2 promotes ubiquitin-mediated degradation of ASK1 to regulate stress responses.
    Sci. Signal., 7, ra8 (2014)


  9. Kawarazaki, Y., Ichijo, H. and Naguro, I.
    Apoptosis Signal-Regulating Kinase 1 (ASK1) as a therapeutic target.
    Expert Opin. Ther, Tar. (review article), 18, 651-664 (2014).


  10. Homma, K., Fujisawa, T., Tsuburaya, N., Yamaguchi, N., Kadowaki, H., Takeda, K., Nishitoh, H.,  Matsuzawa, A., Naguro, I., and Ichijo, H.
    SOD1 as a molecular switch for initiating the homeostatic ER stress response under zinc deficiency.
    Mol. Cell, 52, 75-86 (2013).


  11. Shiizaki, S., Naguro, I. and Ichijo, H.
    Activation mechanisms of ASK1 in response to various stresses and its significance in intracellular signaling.
    Adv. Biol. Regul., (review article), 53, 135-144 (2013).

  12. Naguro, I., Umeda, T., Kobayashi, Y., Maruyama, J., Hattori, K., Shimizu, Y., Kataoka, K., Kim-Mitsuyama, S., Uchida, S., Vandewalle, A., Noguchi, T., Nishitoh, H., Matsuzawa, A., Takeda, K. and Ichijo, H.
    ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney.
    Nat. Commun., 3, 1285 (2012).

  13. Fujisawa, T., Homma, K., Yamaguchi, N., Kadowaki, H., Tsuburaya, N., Naguro, I., Matsuzawa, A., Takeda, K., Takahashi, Y., Goto, J., Tsuji, S., Nishitoh, H. and Ichijo, H.
    A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral sclerosis-linked SOD1 mutants.
    Ann. Neurol., 72, 739-749 (2012).

  14. Sekine,Y., Takagahara,S., Hatanaka, R., Watanabe, T., Oguchi, H., Noguchi, T., Naguro, I., Kobayashi, K., Tsunoda, M., Funatsu, T., Nomura, H., Toyoda, T., Matsuki, N., Kuranaga, E., Miura, M., Takeda, K. and Ichijo,H.
    p38 MAP kinase regulates the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors.
    J. Cell Sci. 124, 3006-3016 (2011).

  15. Takeda, K., Naguro, I., Nishitoh, H.,Matsuzawa, A. and Ichijo, H.
    Apoptosis signaling kinases: from stress response to health outcomes.
    Antioxid. Redox Signal. (review article), 15, 719-761 (2011).

  16. Maruyama, T., Kadowaki, H., Okamoto, N., Nagai, A., Naguro, I., Matsuzawa, A., Shibuya, H., Tanaka, K., Murata, S., Takeda, K., Nishitoh, H. and Ichijo, H.
    CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response.
    EMBO J., 29, 2501-2514 (2010).

  17. Shimizu, Y., Naguro, I., Nishitoh, H. and Ichijo, H.
    ER stress-induced cell death and inflammatory signaling: a therapeutic insight into intractable diseases.
    Endoplasmic Reticulum Stress and Human Disease, (review article), in press.

  18. Hattori, K., Naguro, I., Runchel, C. and Ichijo, H.
    The roles of ASK family proteins in stress responses and diseases.
    Cell Communication and Signaling, (review article), 7, 9 (2009).

  19. Maruyama, J., Naguro, I., Takeda, K. and Ichijo, H.
    Stress-Activated MAP Kinase Cascades in Cellular Senescence.
    Curr. Med. Chem., (review article), 16, 1229-1235 (2009).

  20. Iriyama, T., Takeda, K., Nakamura, H., Morimoto, Y., Kuroiwa, T., Mizukami, J., Umeda, T., Noguchi, T., Naguro, I., Nishitoh, H., Saegusa, K., Tobiume, K., Homma, T., Shimada, Y., Tsuda, H., Aiko, S., Imoto, I., Inazawa, J., Chida, K., Kamei, Y., Kozuma, S., Taketani, Y., Matsuzawa, A. and Ichijo, H.
    ASK1 and ASK2 differentially regulate the counteracting roles of apoptosis and inflammation in tumorigenesis.
    EMBO J., 28, 843-853 (2009).

  21. Noguchi, T., Ishii, K., Fukutomi, H., Naguro, I., Matsuzawa, A., Takeda, K. and Ichijo, H.
    Requirement of reactive oxygen species-dependent activation of ASK1-p38 MAP kinase pathway for extracelluler ATP-induced apoptosis in macrophage.
    J. Biol. Chem., 283, 7657-7665 (2008).

  22. Takeda, K., Noguchi, T., Naguro, I. and Ichijo, H.
    Apoptosis Signal-regulating Kinase 1 (ASK1) in stress and immune response.
    Annu. Rev. Pharmacol. Toxicol., (review article), 48, 199-225 (2008).

  23. Takeda, K., Shimozono, R., Noguchi, T., Umeda, T., Morimoto, Y., Naguro, I., Tobiume, K., Saitoh, M., Matsuzawa, A. and Ichijo, H.
    Apoptosis signal-regulating kinase 2 (ASK2) functions as a MAP3K in a heteromeric complex with ASK1.
    J. Biol. Chem., 282, 7522-7531 (2007).

  24. Yokoyama, U., Minamisawa, S., Adachi-Akahane, S., Akaike, T., Naguro, I., Funakoshi, K., Iwamoto, M., Nakagome, M., Uemura, N., Hori, H., Yokota, S. and Ishikawa, Y. Multiple transcripts of Ca2+ channel alpha1-subunit and a novel spliced variant of the alpha1C-subunit in rat ductus arteriosus.
    Am. J. Physiol. Heart Circ. Physiol., 290, H1660-1670 (2006).

  25. Naguro, I., Adachi-Akahane, S. and Ichijo, H.
    Calcium signaling via voltage-dependent L-type Ca2+ channels.
    Signal Transduction (review article), 4, 195-205 (2004).

  26. Naguro, I., Nagao, T. and Adachi-Akahane, S. Ser(1901) of alpha(1C) subunit is required for the PKA-mediated enhancement of L-type Ca(2+) channel currents but not for the negative shift of activation.
    FEBS Lett., 489, 87-91 (2001).

    論文業績(邦文)

  1. 名黒功:哺乳類細胞におけるシグナリングを対象としたゲノムワイドsiRNAスクリーニング,実験医学増刊「知る・見る・活かす!シグナリング研究2015」一條秀憲、南泰宏 編,羊土社,33(10), 1667-1673, 2015.

  2. 渡邊謙吾、名黒 功、一條秀憲:ハイコンテントゲノムワイドsiRNAスクリーニングによるシグナル伝達研究への応用、実験医学,32(9),1405-1410,2014.

  3. 河原崎陽介、名黒功、一條秀憲:酸化ストレス応答キナーゼASK1の機能と疾患へのかかわり,医学のあゆみ,247(9),746-752,2013.

  4. 河野泰秀、名黒功、一條秀憲:癌とシグナル-ストレス応答シグナル(JNK経路)と癌,遺伝子医学MOOK6-シグナル伝達病を知る-,菅村和夫他 編,メディカルドゥ,192-197,2006.

  5. 名黒 功、松沢 厚、藤野悟央、一條秀憲:ASK1シグナリングのRedoxによる制御と自然免疫応答への関与,Annual Review免疫2007,奥村康他編、中外医学社,73-84,2006.

    研究費獲得状況

    平成27〜29年度 科学研究費補助金・基盤研究B 12,900 千円
    平成27〜28年度 科学研究費補助金・萌芽研究 3,000 千円
    平成25〜26年度 科学研究費補助金・萌芽研究 2,800 千円
    平成23〜24年度 科学研究費補助金・若手研究B 3,400 千円
    平成20〜21年度 科学研究費補助金・若手研究B 3,300 千円
    平成18〜19年度 若手スタートアップ 2,620 千円
    平成18年度 文部科学省がん特定 若手支援委員会 若手共同研究 1,000 千円